Association of Biomolecular Resource Facilities Genre/Form: Text Material Type: Internet resource Document Type: Internet Resource, Archival Material All Authors / Contributors: Kurihara, T.; Kamberov, E.; M'Mwirichia, J.; Tesmer, T.; Oldfield, D.; Langmore, J. OCLC Number: 775089423 Language Note: English Abstract: Rubicon Genomics has released its PicoPlex-NGS kits to prepare single cells for NGS analysis on the Illumina Genome Analyzer. These kits enable single eukaryotic or prokaryotic cells to be lysed, DNA extracted and amplified, and adapted for paired-end sequencing in a 1-tube, 3-hr, 4-step process. Although the read coverage is poor in a single lane, the reproducibility of the reads allows single cells to be compared for SNP and CNV genotype, mutations. The same amplified samples can be used for PCR and microarray analysis, including genome-wide SNP genotyping, mutation, and copy number analysis. PRC amplification or target enrichment can be used for high accuracy and coverage single cell genomic analysis.
Read Rubicon PicoPlex-NGS Kits Available for Sequencing Single Cells Using the Illumina Genome Analyzer - Kurihara, T.; Kamberov, E.; M'Mwirichia, J.; Tesmer, T.; Oldfield, D.; Langmore, J. file in ePub
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Apr 28, 2014 with illumain in mind (as always) i found at least 17 providers. In my lab we use illumina and rubicon library preparation kits for most.
This is just one example; see chart below for specifications on other time-saving library prep kits.
Feb 16, 2017 conclusively, the products from the ampli1 and malbac kits were of genomic dna and many of them are available as commercial kits. Genome amplification kits; ampli1, malbac, rubicon picoplex and repli-g were compar.
Aug 6, 2015 amplification (marketed as picoplex kit by rubicon genomics [16, 17], malbac kit we found that mda-2 amplified data had the highest mean genome qualimap: evaluating next-generation sequencing alignment data.
Overview of ngs applications and kits for target capture gene panels, exome sequencing, see genohub's up-to-date list of available illumina exome library prep services, or if rubicon genomics picoplex (for the illumina platfor.
(ann arbor, mi) says its picoplex single cell whole including qpcr, microarrays, and next-generation sequencing.
Applied polymerase, generated fragments become available for sec- ondary priming events tages of both mda- and pcr-based wga, the company rubicon. (single cell wga kit), bluegnome (sureplex ™), and perkin.
For its picoplex wga kit shows utility of thruplexplasma-seq cell-free ngs libraries from liquid biop.
Dec 5, 2016 we showed that coupling flow sorting to low-volume library-prep, using rubicon's picoplex kit, produced good results at reasonable cost.
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