Full Download Role of small GPTases and alphavbeta5 Integrin in P. aeruginosa-induced increase in lung permeability - Ganter, M T; Roux, J; Su, G; Lynch, S V; Deutschman, C S; Weiss, Y G; Christiaans, S C; Myazawa, B; Kipnis, E; Wiener-Kronish, J P; Howard, M; Pittet, J F | PDF Online

Read Online Role of small GPTases and alphavbeta5 Integrin in P. aeruginosa-induced increase in lung permeability - Ganter, M T; Roux, J; Su, G; Lynch, S V; Deutschman, C S; Weiss, Y G; Christiaans, S C; Myazawa, B; Kipnis, E; Wiener-Kronish, J P; Howard, M; Pittet, J F | ePub

American Thoracic Society 2009 Genre/Form: Journal ArticlePeerReviewed Material Type: Internet resource Document Type: Internet Resource, Article All Authors / Contributors: Ganter, M T; Roux, J; Su, G; Lynch, S V; Deutschman, C S; Weiss, Y G; Christiaans, S C; Myazawa, B; Kipnis, E; Wiener-Kronish, J P; Howard, M; Pittet, J F OCLC Number: 797149487 Language Note: English Notes: application/pdf Abstract: Pseudomonas aeruginosa is an opportunistic pathogen that can cause severe pneumonia associated with airspace flooding with protein-rich edema in critically ill patients. The type III secretion system is a major virulence factor and contributes to dissemination of P. aeruginosa. However, it is still unknown which particular bacterial toxin and which cellular pathways are responsible for the increase in lung endothelial permeability induced by P. aeruginosa. Thus, the first objective of this study was to determine the mechanisms by which this species causes an increase in lung endothelial permeability. The results showed that ExoS and ExoT, two of the four known P. aeruginosa type III cytotoxins were primarily responsible for bacterium-induced increases in protein permeability across the lung endothelium via an inhibition of Rac1 and an activation of the RhoA signaling pathway. In addition, inhibition of the alphavbeta5 integrin, a central regulator of lung vascular permeability, prevented these P. aeruginosa-mediated increases in albumin flux due to endothelial permeability. Finally, prior activation of the stress protein response or adenoviral gene transfer of the inducible heat shock protein Hsp72 also inhibited the damaging effects of P. aeruginosa on the barrier function of lung endothelium. Taken together, these results demonstrate the critical role of the RhoA/alphavbeta5 integrin pathway in mediating P. aeruginosa-induced lung vascular permeability. Additionally, activation of the stress protein response with pharmacologic inhibitors of Hsp90 may protect lungs against P. aeruginosa-induced permeability changes.

Title : Role of small GPTases and alphavbeta5 Integrin in P. aeruginosa-induced increase in lung permeability
Author : Ganter, M T; Roux, J; Su, G; Lynch, S V; Deutschman, C S; Weiss, Y G; Christiaans, S C; Myazawa, B; Kipnis, E; Wiener-Kronish, J P; Howard, M; Pittet, J F
Language : en
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Uploaded : Apr 12, 2021

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